GeneLab Test

RR1 is a validation flight to evaluate the hardware, operational and science capabilities of the Rodent Research Project on the ISS. RNA, DNA, and protein were purified from 14 livers from RR1 mice (female, C57Bl6/J, 16wk old at time of launch), including seven from the Flight group and seven from the Ground Control group. From each group, two liver samples were collected immediately after euthanasia (Flight mice dissected on-orbit after total 37 days after launch). An additional five samples from each group were collected from frozen carcasses dissected post-flight. RNAseq, whole genome BS-seq and proteomic expression profiling were performed.