The NASA Autonomous Mission Operations (AMO) project conducted an experiment to turn over operation and management of selected International Space Station (ISS) systems to the on-board crew. ISS crews managed two different spacecraft systems: the Total Organic Carbon Analyzer (TOCA), a water quality analyzer, and Station Support Computers (SSC) laptops, which are non-critical crew computer systems. These systems were selected because they are representative of systems a future crew may need to operate autonomously during a deep space mission. The crew autonomously operated these systems, taking on mission operations functions traditionally performed by support teams on the ground, using new software tools that provide decision support algorithms for planning, monitoring and fault management, hardware schematics, system briefs, and data displays that are normally unavailable to the crew. The experiment lasted seven months, during which ISS crews managed TOCA and SSCs on 22 occasions. The AMO software processed data from TOCA and SSCs continuously during this seven month period. The combined performance of the software and crew achieved a 88% success rate on managing TOCA activity, the system for which ground-truth was available.